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| physical properties of cytochrome C | | Published by: jane 2009-01-09 |
| | Would like to have your comment how the "electric charge" of
cytochrome C can help in purifying this protein. What does this
electric charge have to do in helping to purify this protein. I know
it is a physical property, but please comment on how it helps in this
purification of cytochrome C. Among many other proteins in the "mix",
how does the "electric charge" identifies Cytochrome C against all the
others.
Ted.
Most proteins carry a negative charge at neutral pH. Cytochrome C
differs in that it carries a positive charge. This aids in separation
from other proteins through ion exchange chromatography, wherein in
this particular case a column packed with a media carrying a negative
charge is used to hold Cytochrome C until is it eluted with a
concentrated salt solution.
Here is a link to an protocol that details purification of cytochrome
C from bovine heart muscle utilizing various separation techniques.
These procedures could easily be applied to a protein mixture as well.
http://www.science.smith.edu/departments/Biochem/Biochem_353/cytoprep.html A Cytochrome c Oxidase Model Catalyzes Oxygen to Water Reduction ::
During the final stage of respiration, cytochrome c oxidase (CcO) catalyzes the .. the physical properties of redox molecules within the SAM (40, 41).
http://www.sciencemag.org/cgi/content/full/315/5818/1565?maxtoshow=&HITS=10&hits=10&RESULTFORMAT=&fulltext=Collman&searchid=1&FIRSTINDEX=0&resourcetype=HWCITHOME |
HistCite - node 844: Structural basis for the molecular properties ::
Title, Structural basis for the molecular properties of cytochrome c(6). Source, BIOCHEMISTRY 41 (50): 14689-14699. Date, 2002 DEC 17
http://garfield.library.upenn.edu/histcomp/merchant-s_citing/node/843.htmlHOME |
Valid 11/2002 - 01/2003 Sigma Chemical Co. P.O. Box 14508 St ::
File Format: PDF/Adobe Acrobat - View as HTMLTO THE BEST OF OUR KNOWLEDGE, THE CHEMICAL, PHYSICAL, AND. TOXICOLOGICAL PROPERTIES HAVE NOT BEEN THOROUGHLY INVESTIGATED. RTECS #: HA5365000. CYTOCHROME C
http://web.ncifcrf.gov/rtp/LASP/intra/forms/msds/msds_cytochrome-c.pdfHOME |
Q TRENNER, KINETICS AND PROPERTIES OF CYTOCHROME C PHOTOOXIDASE OF ::
physical properties. of the. coen-. zyme Q family of compounds. Biochim. BISHOP ET AL-PROPERTIES OF CYTOCHROME C. PHOTOOXIDASE. The stimulation
http://www.plantphysiol.org/cgi/reprint/34/5/551.pdfHOME |
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